Cellular heterogeneity is key principle in biology that can be linked to individual health status of humans. To investigate the ratio of early, normal and late as well as non-responding cells following exposure to chemicals, nanomaterials and bioactive substances, we are developing a single-cell microfluidic platform.
Multi-cellular spheroid array
The aim of the project is the generation of multi-cellular spheroids inside microfluidic devices to assess drug diffusivity.
We are currently developing a microfluidic live cell microarray containing integrated optical microsensors to continuously and non-invasively monitor oxygen consumption and acidification rates of various cell types in the absence and presence of nanoparticles.
Microfluidic wound healing & migration assay
All cell migration and wound healing assays are based on the inherent ability of adherent cells to move into adjacent cell-free areas, thus providing information on cell culture viability, cellular mechanisms and multicellular movements. We have developed a lab-on-a-chip capable of mechanically inducing circular cell-free areas within confluent cell layers.
Functional materials for microfluidic cell cultures
We have developed a novel method for cell cultivation in a micro-sized polymer device under anoxic (absence of oxygen) conditions, where neither additional supplements (oxygen scavengers) nor gas supply is needed.
The dissolved oxygen is scavenged by a modified thiol-ene-epoxy polymer, while the scavenging rate is adjustable by fabrication process and channel geometry.